Protocols

Culturing and microscopy

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Aseptic Technique

Biosafety Cabinet operation

• Understanding the biosafety cabinet
• Preparing for work in the biosafety cabinet
• Working in the biosafety cabinet
• When work is completed

Instructions for preparing media plates

Modified Melin-Norkrans (MMN) media recipe

 

Molecular protocols

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Culture DNA/RNA/Protein extractions

Polymerase Chain Reaction (PCR)

Gel Electrophoresis

Amplicon cleanup and sequencing prep

 

Chemical protocols

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Litter chemistry

Extracellular enzyme assays

• Pipet training
• Soil
• Litter
• Ectomycorrhizal root tips
• Cultures

Ergosterol for total fungal biomass