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Bhatnagar lab of Microbial Ecology

Department of Biology, Boston University

Ramaria in New Hampshire

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Protocols

Culturing and microscopy


Aseptic Technique

Biosafety Cabinet operation

  • Understanding the biosafety cabinet
  • Preparing for work in the biosafety cabinet
  • Working in the biosafety cabinet
  • When work is completed

Instructions for preparing media plates

Modified Melin-Norkrans (MMN) media recipe

 

Molecular protocols


Culture DNA/RNA/Protein extractions

Polymerase Chain Reaction (PCR)

Gel Electrophoresis

Amplicon cleanup and sequencing prep

 

Chemical protocols


Litter chemistry

Extracellular enzyme assays

  • Pipet training
  • Soil
  • Litter
  • Ectomycorrhizal root tips
  • Cultures

Ergosterol for total fungal biomass

 

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Recent Posts

Best Undergraduate Research Award

Posted on May 4, 2023 by jennymbhatnagar
Reply

Congratulations to Izzy for winning the Best Undergraduate Research Award for EBE in the Department of Biology this yeaer!

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Posted in Uncategorized | Leave a reply
  1. Engineering the environmental microbiome Leave a reply
  2. Invasive Ectomycorrhizal fungi in New Phytologist Leave a reply
  3. FICUS award for CCASE project! Leave a reply

Contact information

Department of Biology
Boston University
5 Cummington Street
Boston, MA 02215

Lab phone: 617-353-2346

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